Immortalized MH-S cells lack defining features of primary alveolar macrophages and do not support mouse pneumovirus replication
TA Brenner, TA Rice, ED Anderson, CM Percopo… - Immunology letters, 2016 - Elsevier
Abstract The SV-40-transformed MH-S cell line maintains some, but not all, features of
primary alveolar macrophages (AMs) from BALB/c mice. We show here that MH-S cells
produce inflammatory cytokines IL-6 and CXCL10 in response to challenge with Gram-
positive Lactobacillus reuteri, and to TLR2 and NOD2 ligands Pam3CSK4 and MDP,
respectively. In contrast, although wild-type AMs are infected in vivo by pneumonia virus of
mice (PVM), no virus replication was detected in MH-S cells. Interestingly, the surface …
primary alveolar macrophages (AMs) from BALB/c mice. We show here that MH-S cells
produce inflammatory cytokines IL-6 and CXCL10 in response to challenge with Gram-
positive Lactobacillus reuteri, and to TLR2 and NOD2 ligands Pam3CSK4 and MDP,
respectively. In contrast, although wild-type AMs are infected in vivo by pneumonia virus of
mice (PVM), no virus replication was detected in MH-S cells. Interestingly, the surface …