Mycobacterium tuberculosis CDC 1551, a highly immunogenic outbreak strain, previously
reported to have unique surface distribution of capsular polysaccharide, was used to
generate novel monoclonal antibodies (mabs) to surface mycobacterial targets. Two
immunoglobulin G1 (IgG1) mAbs, 16a1 and 16a6 were generated. The mAbs originated
from the same B cell, bound strongly to whole cell M. tuberculosis CDC1551 and to its cell
wall, membrane and cytosol fractions recognizing a 90kDa protein. Immunoprecipitation …