Identifying vulnerable pathways in Mycobacterium tuberculosis by using a knockdown approach
P Carroll, MC Faray-Kele, T Parish - Applied and environmental …, 2011 - Am Soc Microbiol
P Carroll, MC Faray-Kele, T Parish
Applied and environmental microbiology, 2011•Am Soc MicrobiolWe constructed recombinant strains of Mycobacterium tuberculosis in which expression of
specific genes was downregulated to identify vulnerable drug targets. Growth phenotypes in
macrophages and culture were used to rank targets: the dprE1, clpP1, and fadD32 operons
were the best targets and glnA1, glnE, pknL, regX3, and senX3 were poor targets.
specific genes was downregulated to identify vulnerable drug targets. Growth phenotypes in
macrophages and culture were used to rank targets: the dprE1, clpP1, and fadD32 operons
were the best targets and glnA1, glnE, pknL, regX3, and senX3 were poor targets.
Abstract
We constructed recombinant strains of Mycobacterium tuberculosis in which expression of specific genes was downregulated to identify vulnerable drug targets. Growth phenotypes in macrophages and culture were used to rank targets: the dprE1, clpP1, and fadD32 operons were the best targets and glnA1, glnE, pknL, regX3, and senX3 were poor targets.
American Society for Microbiology