[HTML][HTML] A novel lipase belonging to the hormone-sensitive lipase family induced under starvation to utilize stored triacylglycerol in Mycobacterium tuberculosis
Twenty-four putative lipase/esterase genes of Mycobacterium tuberculosis H37Rv were
expressed in Escherichia coli and assayed for long-chain triacylglycerol (TG) hydrolase
activity. We show here that the product of Rv3097c (LIPY) hydrolyzed long-chain TG with
high specific activity. LIPY was purified after solubilization from inclusion bodies; the enzyme
displayed a K m of 7.57 mm and V max of 653.3 nmol/mg/min for triolein with optimal activity
between pH 8.0 and pH 9.0. LIPY was inhibited by active serine-directed reagents and was …
expressed in Escherichia coli and assayed for long-chain triacylglycerol (TG) hydrolase
activity. We show here that the product of Rv3097c (LIPY) hydrolyzed long-chain TG with
high specific activity. LIPY was purified after solubilization from inclusion bodies; the enzyme
displayed a K m of 7.57 mm and V max of 653.3 nmol/mg/min for triolein with optimal activity
between pH 8.0 and pH 9.0. LIPY was inhibited by active serine-directed reagents and was …