[HTML][HTML] High-throughput mass cytometry staining for immunophenotyping clinical samples
EM Thrash, K Kleinsteuber, ES Hathaway, M Nazzaro… - STAR protocols, 2020 - Elsevier
EM Thrash, K Kleinsteuber, ES Hathaway, M Nazzaro, E Haas, FS Hodi, M Severgnini
STAR protocols, 2020•ElsevierAs mass cytometry (MC) is implemented in clinical settings, the need for robust, validated
protocols that reduce batch effects between samples becomes increasingly important. Here,
we present a streamlined MC workflow for high-throughput staining that generates
reproducible data for up to 80 samples in a single experiment by combining reference
sample spike-in and palladium-based mass-tag cell barcoding. Although labor intensive,
this workflow decreases experimental variables and thus reduces technical error and …
protocols that reduce batch effects between samples becomes increasingly important. Here,
we present a streamlined MC workflow for high-throughput staining that generates
reproducible data for up to 80 samples in a single experiment by combining reference
sample spike-in and palladium-based mass-tag cell barcoding. Although labor intensive,
this workflow decreases experimental variables and thus reduces technical error and …
Summary
As mass cytometry (MC) is implemented in clinical settings, the need for robust, validated protocols that reduce batch effects between samples becomes increasingly important. Here, we present a streamlined MC workflow for high-throughput staining that generates reproducible data for up to 80 samples in a single experiment by combining reference sample spike-in and palladium-based mass-tag cell barcoding. Although labor intensive, this workflow decreases experimental variables and thus reduces technical error and mitigates batch effects.
Elsevier