[HTML][HTML] Biochemical mechanism of lipid-induced impairment of glucose-stimulated insulin secretion and reversal with a malate analogue
A Boucher, D Lu, SC Burgess… - Journal of Biological …, 2004 - ASBMB
Hyperlipidemia appears to play an integral role in loss of glucose-stimulated insulin
secretion (GSIS) in type 2 diabetes. This impairment can be simulated in vitro by chronic
culture of 832/13 insulinoma cells with high concentrations of free fatty acids, or by study of
lipid-laden islets from Zucker diabetic fatty rats. Here we show that impaired GSIS is not a
simple result of saturation of lipid storage pathways, as adenovirus-mediated
overexpression of a cytosolically localized variant of malonyl-CoA decarboxylase in either …
secretion (GSIS) in type 2 diabetes. This impairment can be simulated in vitro by chronic
culture of 832/13 insulinoma cells with high concentrations of free fatty acids, or by study of
lipid-laden islets from Zucker diabetic fatty rats. Here we show that impaired GSIS is not a
simple result of saturation of lipid storage pathways, as adenovirus-mediated
overexpression of a cytosolically localized variant of malonyl-CoA decarboxylase in either …