The reaction mechanism of glutathione reductase from human erythrocytes
GEJ Staal, C Veeger - Biochimica et Biophysica Acta (BBA)-Enzymology, 1969 - Elsevier
1. The reaction mechanism of glutathione reductase (NAD (P) H: oxidized glutathione
oxidoreductase, EC 1.6. 4.2) was studied. By varying the GSSG concentration, a series of
parallel lines was obtained in the 1 v vs 1/[NADPH] plots. NADP+ is a competitive inhibitor
towards NADPH and a noncompetitive one towards GSSG. Both GSSG and NADPH were
inhibitory at high concentrations. In the 1 v vs. 1/[GSSG] plots only parallel lines were
obtained at low NADPH concentrations. The maximum velocity was dependent on the Na+ …
oxidoreductase, EC 1.6. 4.2) was studied. By varying the GSSG concentration, a series of
parallel lines was obtained in the 1 v vs 1/[NADPH] plots. NADP+ is a competitive inhibitor
towards NADPH and a noncompetitive one towards GSSG. Both GSSG and NADPH were
inhibitory at high concentrations. In the 1 v vs. 1/[GSSG] plots only parallel lines were
obtained at low NADPH concentrations. The maximum velocity was dependent on the Na+ …