[HTML][HTML] Comparing pooled peptides with intact protein for accessing cross-presentation pathways for protective CD8+ and CD4+ T cells
H Zhang, H Hong, D Li, S Ma, Y Di, A Stoten… - Journal of biological …, 2009 - ASBMB
To better understand the mechanisms of intracellular trafficking and presentation of
exogenous peptides by antigen-presenting cells (APC), we compared the handling of
overlapping 24-mer peptides from HIV Nef either mixed or covalently linked in tandem in
one protein. Once internalized, peptides trafficked not only to endosomes but also to cytosol,
and activated CD8+ and CD4+ T cells. In contrast, whole protein was found to traffic only to
the endosomal compartments, and primarily activated CD4+ T cells. Finally, with adjuvant …
exogenous peptides by antigen-presenting cells (APC), we compared the handling of
overlapping 24-mer peptides from HIV Nef either mixed or covalently linked in tandem in
one protein. Once internalized, peptides trafficked not only to endosomes but also to cytosol,
and activated CD8+ and CD4+ T cells. In contrast, whole protein was found to traffic only to
the endosomal compartments, and primarily activated CD4+ T cells. Finally, with adjuvant …