Kininogens, the high molecular weight precursor of vasoactive kinins, bind to a wide variety
of cells in a specific, reversible, and saturable manner. The cell docking sites have been
mapped to domains D3 and D5 H of kininogens; however, the corresponding cellular
acceptor sites are not fully established. To characterize the major cell binding sites for
kininogens exposed by the endothelial cell line EA. hy926, we digested intact cells with
trypsin and other proteases and found a time-and concentration-dependent loss of 125 I …