[HTML][HTML] The cyclin-dependent kinase inhibitor p27Kip1 is stabilized in G0 by Mirk/dyrk1B kinase
X Deng, SE Mercer, S Shah, DZ Ewton… - Journal of Biological …, 2004 - ASBMB
Elevated levels of the cyclin-dependent kinase (CDK) inhibitor p27 block the cell in G 0/G 1
until mitogenic signals activate G 1 cyclins and initiate proliferation. Post-translational
regulation of p27 by different phosphorylation events is critical in allowing cells to proceed
through the cell cycle. We now demonstrate that the arginine-directed kinase, Mirk/dyrk1B, is
maximally active in G 0 in NIH3T3 cells, when it stabilizes p27 by phosphorylating it at Ser-
10. The phospho-mimetic mutant p27-S10D was more stable, and the non-phosphorylatable …
until mitogenic signals activate G 1 cyclins and initiate proliferation. Post-translational
regulation of p27 by different phosphorylation events is critical in allowing cells to proceed
through the cell cycle. We now demonstrate that the arginine-directed kinase, Mirk/dyrk1B, is
maximally active in G 0 in NIH3T3 cells, when it stabilizes p27 by phosphorylating it at Ser-
10. The phospho-mimetic mutant p27-S10D was more stable, and the non-phosphorylatable …
