[HTML][HTML] Calmodulin binding and inhibition of cardiac muscle calcium release channel (ryanodine receptor)
DM Balshaw, L Xu, N Yamaguchi, DA Pasek… - Journal of Biological …, 2001 - ASBMB
Metabolically 35 S-labeled calmodulin (CaM) was used to determine the CaM binding
properties of the cardiac ryanodine receptor (RyR2) and to identify potential channel
domains for CaM binding. In addition, regulation of RyR2 by CaM was assessed in [3 H]
ryanodine binding and single-channel measurements. Cardiac sarcoplasmic reticulum
vesicles bound approximately four CaM molecules per RyR2 tetramer in the absence of Ca
2+; in the presence of 100 μmCa 2+, the vesicles bound 7.5 CaM molecules per tetramer …
properties of the cardiac ryanodine receptor (RyR2) and to identify potential channel
domains for CaM binding. In addition, regulation of RyR2 by CaM was assessed in [3 H]
ryanodine binding and single-channel measurements. Cardiac sarcoplasmic reticulum
vesicles bound approximately four CaM molecules per RyR2 tetramer in the absence of Ca
2+; in the presence of 100 μmCa 2+, the vesicles bound 7.5 CaM molecules per tetramer …