Plasma membrane-associated proteins with the ability to partially inhibit perforin-mediated lysis
DM Ojeius, S Muller, CS Hasselkus-Light, JDE Young… - Immunology letters, 1991 - Elsevier
Cytolytic lymphocytes have previously been reported to be resistant to the lytic effects of
perforin. In this work, plasma membrane proteins from a CTL cell line were fractionated by
HPLC, and the eluted fractions were collected based on their ability to inhibit perforin-
mediated hemolysis. Three proteins with inhibitory activity were thus purified, the serine
esterase MCSP-3/granzyme F and the histones H2B and H3. A commercial source of H2B
was able to potently inhibit perforin-mediated lysis, and it was confirmed by FACS analysis …
perforin. In this work, plasma membrane proteins from a CTL cell line were fractionated by
HPLC, and the eluted fractions were collected based on their ability to inhibit perforin-
mediated hemolysis. Three proteins with inhibitory activity were thus purified, the serine
esterase MCSP-3/granzyme F and the histones H2B and H3. A commercial source of H2B
was able to potently inhibit perforin-mediated lysis, and it was confirmed by FACS analysis …
